Space shuttle Endeavor safely landed at Kennedy Space Center in Florida at 10:48 a.m. EDT, Friday.
"Congratulations on a superb mission from beginning to end. Very well done," Mission Control radioed.
"We're happy to be home," replied commander Mark Polansky.
NASA said all objectives of the 16-day mission were met.
The seven astronauts made five space walks and completed additions to the $1 billion international space station.
While there, the crew met up with six other astronauts already working on the lab, which became the biggest gathering ever in space.
The shuttle mission lasted 16 days and spanned 6.5 million miles, one of NASA's longest.
Seven shuttle flights remain to finish the space station, now 83 percent complete with nearly 700,000 pounds of mass. The next launch, by Discovery, is targeted for the end of August.