More than a dozen same-sex couples have already been married in the nation's capital and at least 100 others have received marriage licenses.
Same-sex weddings started in Washington, D.C., Tuesday. Meanwhile, gay marriage opponents are still fighting in court to let residents vote on the issue.
"If people get married now, then you're going to have the same mess that they had in California where thousands of people were married and then when it got on the ballot, they repealed everything," Bishop Harry Jackson, with Stand4Marriage, said. "It was really not the best."
CBN News spoke with Janice Crouse of Concerned Women for America about the opposition to same-sex marriage in D.C. and why so many are upset. Click play to watch.
"I believe this is a beginning of a national offensive to make gay marriage the law of the land," he said.
Despite last-minute appeals from traditional marriage supporters, the Supreme Court did not stop the recently signed law from going into effect March 3.
The District of Columbia is the sixth place in the country to legalize same-sex marriage, joining Connecticut, Iowa, Massachusetts, New Hampshire and Vermont.