The Farsi Christian News Network reports that Islamic security agents entered the church premises, arrested everyone present, and confiscated church materials.
The detained are being held in an undisclosed location.
The raid came just days after church elders asked authorities for information about another pastor and his wife who are being detained. They were told the two would be released if the church would shuts its doors.
The Church of the Assembly of God in Tehran started as a house church in 1995 and has been building up other house churches in the city.